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    10X Genomics data analysis spatial transcriptomic experiment
    Data Analysis Spatial Transcriptomic Experiment, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analysis+spatial+transcriptomics/pm42226209-88-10-38
    Average 86 stars, based on 1 article reviews
    data analysis spatial transcriptomic experiment - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Multi-modal refinement of the human heart atlas during the first gestational trimester
    Article Snippet: As transcriptomics of single nuclei is high-resolution but sparse, and removes informative positional information from samples, we also undertook 10x Genomics Visium spatial transcriptomics analysis on two sections from a male heart collected at 8.4 pcw and four sections from a female one at 9.7 pcw ( , Figs. S4-9).

    DNA Sequencing:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Mutagenesis:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Gene Expression:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Article Title: Spatial transcriptomics implicates the thalamus and cortex in autism and schizophrenia
    Article Snippet: We mounted 4 such sections from 1 brain on a Visium Spatial Gene Expression Slide (10x Genomics, 1000187) and stored at -80°C until further processing. .. Spatial transcriptomics analysis was performed using the 10x Genomics Visium Spatial Gene Expression Assay. ..

    Article Title: Spatial lipidomics reveals sphingolipid metabolism as anti-fibrotic target in the liver.
    Article Snippet: .. Analysis of published transcriptomics data Processed spatial transcriptomics data (median-normalized average gene expression; Visium, 10X Genomics) comparing parenchymal and fibrotic liver regions from 8 patients with cirrhosis were downloaded (19). ..

    RNA sequencing:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Expressing:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Spatial Transcriptomics:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Article Title: Spatial transcriptomics implicates the thalamus and cortex in autism and schizophrenia
    Article Snippet: We mounted 4 such sections from 1 brain on a Visium Spatial Gene Expression Slide (10x Genomics, 1000187) and stored at -80°C until further processing. .. Spatial transcriptomics analysis was performed using the 10x Genomics Visium Spatial Gene Expression Assay. ..

    Article Title: IFITM3-MET interaction drives osimertinib resistance through AKT pathway activation in EGFR -mutant non–small cell lung cancer
    Article Snippet: .. Spatial transcriptomics analysis was performed with the 10X Genomics Visium HD platform. ..

    Article Title: IFITM3-MET interaction drives osimertinib resistance through AKT pathway activation in EGFR-mutant non-small cell lung cancer.
    Article Snippet: .. Spatial transcriptomics analysis was performed with the 10X Genomics Visium HD platform. ..

    Article Title: Spatial lipidomics reveals sphingolipid metabolism as anti-fibrotic target in the liver.
    Article Snippet: .. Analysis of published transcriptomics data Processed spatial transcriptomics data (median-normalized average gene expression; Visium, 10X Genomics) comparing parenchymal and fibrotic liver regions from 8 patients with cirrhosis were downloaded (19). ..

    Article Title: Spatial multiomics dissects the SARS-CoV-2-induced disruption of cell adhesion and immune dynamics in the human hippocampus.
    Article Snippet: .. Xenium in situ spatial transcriptomics To precisely map the spatial transcriptomic landscape within the human HP–EC following SARS-CoV-2 infection, we performed Xenium in situ spatial transcriptomic analysis (10x Genomics, USA) on 2 human HP–EC samples that exhibited strong SARS-CoV-2 S protein signals in prior mIHC staining. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Immunofluorescence:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    In Vitro:

    Article Title: Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma.
    Article Snippet: .. STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS B Glioblastoma patient samples B Establishment and propagation of patient-derived cancer stem-like cells B Establishment of patient-derived tumor-associated macrophages d METHOD DETAILS B 5-ALA administration and patient sample collection B Genomic DNA sequencing B Mutation, copy-number and gene expression data B Single-nucleus RNA-sequencing B Definition of malignant cells B Cellular dynamics B Gene regulatory networks B Identification of differentially expressed genes B Expression analysis of the SCENIC-identified ZEB1 targets B Cell-cell interaction analysis B Pathway and MacSpectrum analyses B Spatial transcriptomics B 10x Genomics Visium analysis B Definition of malignant spot B 10x Visium spot deconvolution B 10x Genomics Visium sample clustering B 10x Genomics Visium spot clustering and intracellular communica- tion B 10x Visium microglia spatial correlation B CD11b enrichment B IL-1b and Wnt-5a ELISA B IL-1RAcP and Frizzled-3 immunofluorescence B In vitro treatment assay B Survival analysis d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2024.115149. ..

    Transcriptomics:

    Article Title: Spatial lipidomics reveals sphingolipid metabolism as anti-fibrotic target in the liver.
    Article Snippet: .. Analysis of published transcriptomics data Processed spatial transcriptomics data (median-normalized average gene expression; Visium, 10X Genomics) comparing parenchymal and fibrotic liver regions from 8 patients with cirrhosis were downloaded (19). ..

    In Situ:

    Article Title: Spatial multiomics dissects the SARS-CoV-2-induced disruption of cell adhesion and immune dynamics in the human hippocampus.
    Article Snippet: .. Xenium in situ spatial transcriptomics To precisely map the spatial transcriptomic landscape within the human HP–EC following SARS-CoV-2 infection, we performed Xenium in situ spatial transcriptomic analysis (10x Genomics, USA) on 2 human HP–EC samples that exhibited strong SARS-CoV-2 S protein signals in prior mIHC staining. ..

    Infection:

    Article Title: Spatial multiomics dissects the SARS-CoV-2-induced disruption of cell adhesion and immune dynamics in the human hippocampus.
    Article Snippet: .. Xenium in situ spatial transcriptomics To precisely map the spatial transcriptomic landscape within the human HP–EC following SARS-CoV-2 infection, we performed Xenium in situ spatial transcriptomic analysis (10x Genomics, USA) on 2 human HP–EC samples that exhibited strong SARS-CoV-2 S protein signals in prior mIHC staining. ..

    Staining:

    Article Title: Spatial multiomics dissects the SARS-CoV-2-induced disruption of cell adhesion and immune dynamics in the human hippocampus.
    Article Snippet: .. Xenium in situ spatial transcriptomics To precisely map the spatial transcriptomic landscape within the human HP–EC following SARS-CoV-2 infection, we performed Xenium in situ spatial transcriptomic analysis (10x Genomics, USA) on 2 human HP–EC samples that exhibited strong SARS-CoV-2 S protein signals in prior mIHC staining. ..



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    Spatial Transcriptomics Inc lung cancer research immune network technology data analysis clinical application spatial transcriptomics • merfish
    a Schematic diagram of the spatial <t>transcriptome</t> analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.
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    a Schematic diagram of the spatial <t>transcriptome</t> analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.
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    a Schematic diagram of the spatial <t>transcriptome</t> analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.
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    a Schematic diagram of the spatial <t>transcriptome</t> analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.
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    a Schematic diagram of the spatial transcriptome analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.

    Journal: bioRxiv

    Article Title: CXCL10 drives female-specific tau pathology progression and defines sex-dependent vulnerability in tauopathy model mice

    doi: 10.64898/2026.04.19.719088

    Figure Lengend Snippet: a Schematic diagram of the spatial transcriptome analysis using Xenium Prime 5K. Formalin fixed paraffin embedded (FFPE) sections were prepared from 12-month-old Tau Tg mice and age-matched WT controls. b UMAP visualizing the cell cluster detected by Xenium in the brains of Tau Tg and WT mice. Neuronal cells were classified as IT (intratelencephalic), ET (extratelencephalic), Glut (glutamatergic), NP (near-projecting), CT (corticothalamic), L6b (layer 6b), DG (dentate gyrus), IMN (immature neurons), CTX (cerebral cortex), CGE (caudal ganglionic eminence), GABA (GABAergic), MGE (medial ganglionic eminence), CNU (cerebral nuclei), LGE (lateral ganglionic eminence), Hya (anterior hypothalamic), HY (hypothalamus), MM (medial mammillary nucleus), LH (lateral habenula), TH (thalamus), MB (midbrain), HB (hindbrain), Sero (serotonergic), MY (medulla), NN (non-neuronal), NP (near-projecting), OB (olfactory bulb), OEC (olfactory ensheathing cells), and OLF (olfactory areas). c Cxcl10 mRNA signal was plotted using Feature Plot on UMAP. d Quantitative Cxcl10 gene expression using violin plots in AC-Epen, BAM, DG-IMN Glut, IT-ET Glut, MG, and T cell types. e, h Representative plots of the result of re-clustering AC-Epen ( e ) and immune cluster ( h ), respectively. f Plots of Cxcl10 + cells in the cluster shown in and represented according to genotype. g, j Figures showing spatial distribution of AC8 ( g ) and MG3 ( j ) clusters in the brains of WT and Tau Tg mice. k Representative images of coronal section of mouse brain by Xenium explorer. Scale bar = 1 mm. l Spatial information of Cxcl10 + astrocytes and microglia in the hippocampus of Tau Tg mice using Xenium explorer. Scale bar = 100 μm. Number of mice used: male WT (n = 1), male Tau Tg (n = 1), female WT (n = 1), and female Tau Tg (n = 1). Statistical analysis was performed using a Wilcoxon rank sum U statistic test ( d ). Source data are provided in the Source Data file.

    Article Snippet: FFPE brain sections were analyzed using Xenium spatial transcriptome analysis (10x Genomics).

    Techniques: Formalin-fixed Paraffin-Embedded, Olfactory, Gene Expression